Composite

Part:BBa_K2970003:Design

Designed by: Alexander Bast   Group: iGEM19_Hamburg   (2019-10-02)


Trigger 1 Composition


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The triggers were produced using primers and then inserted with golden gate assembly into the backbone with a strong promoter and terminator. As backbone pSB1C3 was used, from which first BsaI sites were removed and then BsmBI sites were added to cut out the ampicillin resistance later.


Source

References

Green, A.A. et al. "Complex cellular logic computation using ribocomputing devices." (Nature, 2017)